MARGenomics is a core facility from IMIM (Hospital del Mar Medical Research Institute) with more than 10 years of experience. An integrated service is offered to both public and private organizations, providing support to researchers during all the experimental process, from basic research to clinical diagnosis.
The services offered include Next Generation Sequencing (NGS), Microarray processing, quantitative PCR (qPCR) and sample quality assessment. Experienced in difficult samples such as low input or FFPE samples. MARGenomics also provides support in genomics and bioinformatics biomedical research, focusing on omics data analysis. Experimental design consultancy, statistical data analysis and orientation to its biological interpretation are also included.
Offered services
• Experimental design advising
• DNA / RNA /miRNA quality control (quantification and integrity)
• Microarray processing
• NGS processing
• Data quality analysis
• Statistical data analysis, even if data was obtained in an external lab
• Orientation to data interpretation with pathway analysis, Gene Ontology (GO) and specific tools
Equipment
• Affymetrix Research Platform: GCS3000 with autoloader
• Agilent Bioanalyzer 2100
• NanoDrop 2000 Spectrophotometer
• Qubit Fluorometer
Available NGS techniques
• Globin/rRNA depletion (RNASeq)
• Poly-A capture (RNASeq)
• Exome capture (RNASeq, FFPE samples)
• SmallRNASeq (miRNAs from serum/plasma)
• Microbiome Panel
Available arrays
Expression arrays
• Clariom D Human/Mouse/Rat Array
• Clariom S Human/Mouse/Rat Array
• Human/Mouse/Rat Gene 2.0 ST Array
• Human Transcriptome Array 2.0
• miRNA 4.0 Array
• Other species available
Genomic arrays
• CytoScan HD Array
• CytoScan 750K Array
• OncoScan Array
Bioinformatic applications
• RNASeq: quality control, alignment, differential expression analysis, functional analysis (enrichment, GSEA), isoform analysis, alternative splicing detection, population deconvolution, expression signatures
• Expression arrays: quality control, differential expression analysis, functional analysis (enrichment, GSEA), deconvolution, expression signatures
• Genomic arrays: quality control, detection of copy number alterations, loss of heterozygosity (LOH), uniparental disomies (UPD) and mosaicism, virtual molecular karyotype, SNPs genotyping and screening of FFPE samples
• Whole Exome/Genome Sequencing: quality control, alignment, SNP calling, CNA calling, mutational signature analysis
• Other omics: small RNA-Seq, single cell RNA-Seq, metagenomics, qPCR
• Advanced: integration of omic data, statistical analysis, reanalysis of public data (GEO, TCGA), classification and prediction
• Consulting and support: visualization of data, training, data submission public repositories, Manuscript preparation, grant application support
Biostatistical advise
Service offered to give methodological and statistical support to biomedical researchers.
• Protocol design advise
• Sample size estimation
• Database design, handling and quality control
• Statistical analysis
• Advise in communications and publications.
Scientific & Technical Platforms Coordinator: Dra. Núria Somoza Abelló
Telephone: +34 93 3160404
margenomics@imim.es
Data analysis responsible: Dra. Júlia Perera Bel
Telephone: +34 93 3160628
jperera@imim.es
Laboratory responsible: Marta Bódalo Torruella
Telephone: +34 93 3160577
mbodalo@imim.es
Biostatistics responsible: Xavier Duran
Telephone: +34 93 3160614
xduran@imim.es
To make a service request an e-mail must be sent to margenomics@imim.es. In general, a service request follows the following steps:
1. Interview
2. Service request (application user's registry)
3. Cost estimation approval
4. Sample delivery
5. Sample quality control
6. Sample processing (NGS, microarray, qPCR)
7. Data analysis
8. Report delivery
ISO 9001:2015