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Genotyping Core Facility

Genotyping on a medium to high-throughput scale is becoming a powerful tool in animal and plant breeding. Our service offers marker (mainly SNPs and SSRs or microsatellites) development and application for public research projects as well as private breeding programs within a broad spectrum of species. For this purpose we have at our disposal a wide range of genotyping platforms.

 

EQUIPMENT AND APPLICATIONS

Illumina BeadArray Reader

  • Custom multiplex genotyping  (96 to 3072-plex panels)
  • High-throughput genotyping using the portfolio of Illumina Infinium fixed content BeadChips (more than 50,000 probes per Beadchip)

 

Illumina BeadXpress Reader

  • Custom multiplex genotyping (from 48 to 384 SNPs)

 

PSQ HS 96 Pyrosequencing Instrument

  • SNPs and insertion/deletions analysis
  • CpG methylation
  • Allele quantification

 

ABI PRISM 7900HT System

  • Quantification of gene expression levels  (relative or absolute quantification)
  • Quantification of microRNAs.
  • Allelic discrimination studies (SNPs)
  • Pathogen detection and quantification
  • Validation of Copy number variation (CNV)

 

ABI PRISM 3130xl Genetic Analyzer

  • SSR Analysis
  • SNaPshot
  • AFLP

 

Anna Castelló, PhD. (Animal Genotyping) & Werner Howad, PhD. (Plant Genotyping)
Service Managers
+34 935636600
Access Procedures

Users should get in touch with Werner Howad (Plant Genotyping, Werner.Howad@cragenomica.es) or with Anna Castelló (Animal Genotyping, Anna.castello@cragenomica.es), preferentially by email.