You are here

Proteomics Service

Services an features provided: 1) Determination of the molecular mass of proteins and peptides by mass spectrometry (MALDI-TOF). 2) Identification of proteins by peptide mass fingerprint. • In-gel tryptic digestions of 1D Bands and 2D Spots or proteins in solution. • Peptide analysis by MALDI-TOF MS, getting the peptide maps • Identification of proteins by peptide mass fingerprinting searching in databases. 3) Identification of proteins by peptide mass fingerprint and peptide fragmentation by mass spectrometry (MALDI-TOF/TOF). • In-gel tryptic digestions of 1D Bands and 2D Spots or proteins in solution. • Analysis of the peptides by MALDI-TOF MS, getting the peptide maps. • Fragmentation of peptides (1 to 3) (MS / MS) • Search and protein identification by peptide mass fingerprint (MS data) through peptides sequence(s) (MS / MS data), or a combination of both techniques (MS + MS / MS) 4) Separation of proteins by one-dimensional electrophoresis, SDS-PAGE. Electrophoretic technique for separating proteins according to their molecular weight. 5) Separation of complex protein samples using the technique of two-dimensional electrophoresis (2D-PAGE). This technique allows the separation of proteins based on two features: its isoelectric point (Isoelectric focusing, IEF) and molecular weight (electrophoresis under denaturing conditions, SDS-PAGE).

Carme Quero
Head of the Core Facility
+34 934006171
Access Procedures

Contact Carme Quero E-mail: carme.quero@iqac.csic.es Telephone: +34 93 400 6171

Capacity External Users
70%
Type
Mixed