The Proteomics Platform is aimed at the separation, quantification, identification and characterisation of proteins in biological systems by two-dimensional gel electrophoresis, multidimensional liquid chromatography, and mass spectrometry. The unit provides services range from simple protein identification to more complex analysis such as different level of protein expression quantitation and characterization of post translational modifications (PTMs, phosphorylation, acetylation, methylation). The laboratory has a comprehensive system for the separation of proteins by two-dimensional gel electrophoresis. It also has a nano-UPLC system that enables two-dimensional liquid chromatography analysis as a gel-free alternative approach. The unit is equipped with two nano-UPLC liquid chromatographers and three mass spectrometers: a LTQ-Orbitrap Velos, a MALDI-TOF/TOF and Q-TOF. In addition to LC-MS, the unit has an off-line HPLC, a protein picker for the retrieval of proteins from gels and an automatic sample digester.
As a technological platform, the proteomics unit develops its own research projects and participates in collaborative projects with different research groups. Some of the projects the proteomics unit is involved are the sepsis biomarker discovery in blood plasma, and Proteomic study of human neurodegenerative diseases.