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Proteomics and Structural Biology Service (SePBioEs)

The Proteomics and Structural Biology Service (SePBioEs) allows private industry and public entities to access the latest technology in the field of protein production and characterization and related technologies. 

SePBioEs offers its services to researchers and both, public and private entities, by providing our expertise in both R+D+I projects development and those occasional services derived from the use of our facilities. 

The SePBioEs aims to provide expertise in the field of proteomics, protein production and crystallography to support, scientifically and technologically, our clients. The specific objectives are:

-Transfer of technology to companies

-Technological and methodologica development

-To obtain high added value products and substances of biotechnological interest

-Training courses on proteomics, protein production and crystallography techniques applied to productive processes

-Development of biotech products

-Technological advisory service

AVAILABLE TECHNIQUES

MALDI-TOF Mass Spectrometry

  • Molecular weight  determination (proteins, oligonucleotides, sugars...)
  • Protein Profiling
  • Identification of protease inhibitors by Intensity Fading MALDI-TOF
  • Protein identification by Peptide Mass Fingerprint (PMF) and PMF+MS/MS
  • MALDI-imaging (Tissue characterization by MALDI-MS)

ESI-IT Mass Spectrometry

  • LC-MS/MS
  • Protein molecular weight determination
  • Characterization fo post-traductional modification (phosphorilations...)
  • Peptide sequencing

N-terminal Edman Sequencing

  • Protein and peptide sequencing

Chromatography:

  • HPLC chromatography (analitic, micro & nano)
  • FPLC chromatography
  • Atmospheric chromatography

Electrophoresis:

  • 1D Electrophoresis
  • 2D Electrophoresis
  • pI determination, native isolectrofocusing
  • Differential Proteomics (DIGE)
Personnel
David Reverter Cendrós - Director del SeBioEs i cap del l'àrea de Cristal·lografia
Elena Garcia Fruitós - Technical Director of protein production platform
Neus Ferrer Miralles - Scientific Director of protein production platform
Silvia Bronsoms Fabrellas - Technical Director of the Proteomics facility
Sebastian Trejo - Research Technician
David Reverter
Director
+34 935868935
Type
Public