Techniques
- Two-dimensional electrophoresis
- Image analysis (DIGE)
- Mass spectrometry (MALDI-TOF, MS/MS and top-down proteomics)
- Liquid chromatography (HPLC)
Range of services
- Separation of peptides and proteins
- Quantitative analysis of protein samples
- Identification of proteins
- Whole protein analysis
Examples of application
- Study of protein markers
- Study of post-translational modifications in protein samples
- Quantitative analysis
Equipment
- Electrophoresis:
- Amersham Biosciences IPGphor 2-D electrophoresis protein separation system
- Bio-Rad PROTEAN-IEF
- Image analysis:
- Amersham Biosciences DeCyder DIGE enabled TYPHOON TRIO fluorescent scanner
- Scission and digestion of gel stains:
- Genomic Solutions Investigator ProGest digestion unit (up to 96 samples)
- Mass spectrometry and liquid chromatography:
- LTQ-Orbitrap Velos ion trap mass spectrometer with Thermo Finnigan electron transfer dissociation (ETD) accessory
- Thermo Finnigan LCQ DECA XP ESI ion trap
- Applied Biosystems MALDI-TOF Voyager DE perspective mass spectrometer
- Proxeon Nano LC Easy and Eksigent Nano LC Ultra AS2 nano liquid chromatographs
- Data analysis:
- Licences for Protein Prospector, SEQUEST and Proteome Discoverer 1.2 from Thermo
- Finnigan , and PEAKS from BioInformatics Solutions
Institution
Access Procedures
Any person applying for the first time for any services by CCiTUB must enter as a new user. For this purpose it is necessary to fill in an application form which can be downloaded from http://www.ccit.ub.edu/EN/m4.html. The form must be sent to
Keywords
Assurance of quality
ISO 9001:2008. The CCiTUB have well-established a system of management of the quality based on the ISO norm 9001, which also satisfies the Good Manufacturing Practice requirements (GMP).
Recognition by the Food and Drug Administration as a contract laboratory to perform drug tests.
Acknowledged laboratory Register of Agroalimentary Laboratories of Catalonia.