Techniques

• NMR spectroscopy (400-800 MHz) to study samples in solution and observe active nuclei (1H, 13C, 19F, 31P, 113Cd, 195Pt, 29Si, 15N, etc.)
• HR-MAS: NMR spectroscopy to study samples in gel and tissues

Range of services

• Structural determination of organic and inorganic compounds (small-medium molecular weight)
• One- and two-dimensional experiments (COSY, DQFCOSY, TOCSY, HSQC, HMBC, NOESY). DOSY diffusion experiments.
• Combined experiments such as HMQC-TOCSY and other heteronuclear experiments.
• Analysis of complex structures (natural products, compounds of biological interest) Multi-dimensional experiments for structural determination in proteins.
• Combined use of 1H, 13C i 15N for the study of complex molecules such as proteins through two- and threedimensional inverse detection experiments.
• Implementation of new sequences and methods; methodological development and application.
• Control of synthesis processes and/or purification of organic compounds. Determination of impurities and minority components; quantitative analyses.
• HR-MAS experiments for samples in gel or semi-solids; study of metabolites in tissues.
• Process monitoring, kinetic monitoring; variable temperature experiments.
• Determination of interactions between proteins and low-molecular weight compounds
• Work conducted in accordance with standardized procedures (SOP) for the analysis of APIs (USP, European Pharmacopoeia and ASTM standards).
• Identification of compounds, spectral assignment, reports and evaluations.

Examples of application

• NMR spectral assignment for the identification of structures.
• Heparin analysis (in compliance with FDA-USP and European Pharmacopoeia standards).
• Poloxamer analysis (according to USP procedure).
• PGLA polymer analysis (in accordance with ASTM standards).
• Identification analysis for compliance with REACH standard.
• Study of metabolites resulting from the application of an active ingredient (whether directly or through a prior HPLC system treatment).
• Quantitative analysis through the use of internal patterns.
• Determination of the degree of deuteration.
• Purity studies and studies of the composition of soluble polymers. HR-MAS experiments with tissues.
• Application of NMR in the analysis of food products (oils, dairy, juices, additives, etc.).
• Consulting and training for the optimization of the use of existing NMR instrumentation at private centres.

Equipment

• Bruker Avance 800 NMR spectrometer (800 MHz) with cryprobe.
• Bruker Avance 600 NMR spectrometer (600 MHz) with cryprobe.
• Bruker Avance III 600 NMR spectrometer (600 MHz)
• Varian Inova-500 NMR spectrometer (500 MHz)
• Varian VNMRS500 NMR spectrometer (500 MHz)
• Oxford Instruments HyperSense 52-DNP-5089 hyperpolarizer
• Bruker DMX-500 NMR spectrometer (500 MHz) with HR-MAS probe
• Varian VNMRS400 NMR spectrometer (400 MHz)
• Two Varian Mercury-400 NMR spectrometers (400 MHz)
• Varian Gemini-300 NMR spectrometer (300 MHz), only 1H, 13C.