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Molecular interaction analysis

Techniques

  • Surface Plasmon Resonance (SPR), real time and label-free interaction analysis.

Range of services

  • Set up for every pair of molecules: optimization of the ligand immobilization (way and density to be immobilized), reference channel, buffer running and temperature.
  • Optimization of strategies to obtain the best results in accordance the source of the samples: qualitative or quantitative perspectives.
  • Evaluation of the rates constants (kon, koff) and equilibrium constants (KA, KD) in different models: protein/DNA, protein/protein, protein/peptide, carbohydrate/protein, RNA/DNA.
  • Screening of lead candidates in drug discovery.
  • Evaluation of the association and dissociation rates and the half-life complex measurements.
  • Thermodynamic analysis.

Examples of application

  • Determination of the binding domain for the analysis of different mutants against targets.
  • Effect of cofactors, metal ions and other molecules against the binding kinetics.
  • Toxin detection.
  • Characterization of immune response.
  • Effect (in kinetics terms) of different modifications of antibodies.

Equipment

  • Biacore T100 (GE)
Dr. Jaume Comas Riu
Contact person
934 034 654
Access Procedures

Any person applying for the first time for any services by CCiTUB must enter as a new user. For this purpose it is necessary to fill in an application form which can be downloaded from http://www.ccit.ub.edu/EN/m4.html. The form must be sent to .

Assurance of quality

ISO 9001:2008. The CCiTUB have well-established a system of management of the quality based on the ISO norm 9001, which also satisfies the Good Manufacturing Practice requirements (GMP).

  

Recognition by the Food and Drug Administration as a contract laboratory to perform drug tests.

Acknowledged laboratory Register of Agroalimentary Laboratories of Catalonia.