Techniques

• DNA sequencing and fragment analysis by automated capillary electrophoresis.
• Massive parallel sequencing (MPS).
• Real-time PCR (compatible with TDLA 384-well microfluidic cards).
• Magnetic extraction of nucleic acids.
• RNA quality and quantity control.

Range of services

• DNA sequencing and fragment analysis by capillary electrophoresis:

• De novo sequencing
• BAC-end sequencing and fingerprinting
• Mitochondrial DNA sequencing
• Resequencing
• Heterozygote detection
• Epigenetic studies
• Genotyping: SNPs, AFLP, RFLP, LOH, microsatellites (STRs, VNTRs)

• Massive parallel sequencing (MPS):

• Whole genome and transcriptome sequencing
• Resequencing of specific regions
• Differential gene expression
• Genetic variation between individuals
• MicroRNAs
• Epigenetic profiling
• Identification of points of union of DNA- and RNA-related proteins

• Real-time PCR:

• Gene expression and validation of microarray results
• Genotyping and genetic variation (SNPs, ins/del, etc.)
• Methylation
• Viral load testing
• microRNA, siRNA
• Pathogen detection in food and environmental samples
• Presence and absence of transgenes
• Screening by high resolution melting (HRM)

Examples of application

• Sequencing of unknown species
• Human identification: population studies
• Epigenetic studies
• Pathogen detection in food
• Gene expression variations in response to pharmaceuticals
• Magnetic DNA extraction from blood, cell and tissue samples
• Detection of cancer cells in the blood
• Paternity tests

Equipment

Bellvitge Campus

• Applied Biosystems ABI Prism 7900 HT quantitative real-time PCR (TLDA, 384 and 96 wells)
• Chemagen Magnetic nucleic acid isolation module
• Agilent Technologies Bioanalyzer 2100
• BMG Labtech Fluostar Optima Microplate reader with double injection system and temperature control

Diagonal Campus

• Applied Biosystems ABI Prism 3730 capillary electrophoresis DNA sequencer (48 capillary)
• Applied Biosystems ABI Prism 3730XL capillary electrophoresis DNA sequencer (96 capillary)
• Applied Biosystems ABI Prism 7900 HT quantitative real-time PCR (TLDA, 384 and 96 wells block)
• Roche Light Cycler 480 II quantitative real-time PCR (384 and 96 wells block)
• Agilent Technologies Bioanalyzer 2100
• Applied Biosystems thermocyclers: PCR ABI 9700, PCR ABI 9800 Fast, PCR ABI 2400 and 2720; MGW Biotech PCR Primus thermocycler; Techne PCR TC-412 thermocycler
• Beckman Coulter Biomeck NXP robotic workstation
• Beckman Coulter Z series particle counter
• Qiagen Mixer Mill MM 300 for grinding and homogenizing samples