You are here

Flow Cytometry Unit

FLOW CYTOMETRY UNIT

Our Flow Cytometry facility provides users with the state-of-the-art equipment for flow Cytometry analysis and high speed cell sorting. Unit personnel support users for experimental design, data analysis and acquisition.

Following manufacturer instructions, a proper maintenance and standard quality control procedures are routinely applied to ensure best quality results.

The Unit is equipped with 2 flow cytometers:

  • Beckman Coulter High Speed Cell Sorter Moflo-XDP (488nm, 635nm lasers & up to 7 colors [5+2])
     
  • Beckman Coulter Gallios Analyzer (405nm, 488nm, 635nm laser & up to 10 colors [2+5+3])

 

Services are:

  • Sample acquisition at the analyzer, either with technical support, or without, after receiving a proper training.
  • Cell Sorting with technical support.
  • Data analysis support if required.

 

Applications:

The aim of the Cytometry Unit is to get ready and focus on the applications of interest of our users.

Most common applications that routinely run are:

 

Cell Sorting:

  • In purify mode (when the most important is to get a high purity of the cell population of interest from the original sample). Up to 4 cell populations at the same time (4way sorting).
  • In enrich mode (when the most important is to get a high recovery of the cell population of interest from the original sample). So that, purity are slightly compromised. Available in 4 way sorting too.
  • In single cell deposition (to get just one single cell or any desired number of cells within a single well using any of the standard multiwelll plates).
  • In mixed mode (purify and enrich mode run simultaneously even for 2 cell populations at a time).

 

For Analysis:

  • Multicolor Immunophenotyping Characterization.
  • Viability Assays.
  • Cell Cycle and Proliferation Assays.
  • Gene Expression (Fluorescent Protein analysis).
  • Etc…
Personnel

Jose Miguel Andres Vaquero

Cristina Morera Albert

Jose Miguel Andres Vaquero
Head of the Cytometry Unit
+34933160325
Access Procedures

The CMRB Cytometry Unit is located in the 7th floor at the PRBB Building.

If you would like to enquire about a service offered by the CMRB Flow Cytometry Unit, please contact us at cytometry.unit@cmrb.eu.
Only registered users are able to request services through our Core Facility website. If you would like to request a service, please sign up as a user and submit an online request.

Capacity External Users
50%
Type
Public