FLOW CYTOMETRY UNIT

Our Flow Cytometry facility provides users with the state-of-the-art equipment for flow Cytometry analysis and high speed cell sorting. Unit personnel support users for experimental design, data analysis and acquisition.

Following manufacturer instructions, a proper maintenance and standard quality control procedures are routinely applied to ensure best quality results.

The Unit is equipped with 3 flow cytometers:

·         1x Beckman Coulter High Speed Cell Sorter Moflo-XDP (488nm, 635nm lasers & up to 7 colors [5+2])
·         2x Beckman Coulter Gallios Analyzer (405nm, 488nm, 635nm laser & up to 10 colors [2+5+3])

Services are:

• Sample acquisition at the analyzer, either with technical support, or without, after receiving a proper training.
• Cell Sorting (from 1 to 4 cell populations at a time)
  • Purify Mode.
  • Enrich Mode.
  • Single Cell Deposition
• Analysis:
  • Multicolor Immunophenotyping.
  • Viability Assays.
  • Cell Cycle & Proliferation Assays.
  • Gene Expression (Fluorescent Protein Analysis).
  • Etc…
• Data analysis support if required

Applications:

The aim of the Cytometry Unit is to get ready and focus on the applications of interest of our users.

Most common applications that routinely run are:

Cell Sorting:

• In purify mode (when the most important is to get a high purity of the cell population of interest from the original sample). Up to 4 cell populations at the same time (4way sorting).

• In enrich mode (when the most important is to get a high recovery of the cell population of interest from the original sample). So that, purity are slightly compromised. Available in 4 way sorting too.

• In single cell deposition (to get just one single cell or any desired number of cells within a single well using any of the standard multiwelll plates).

• In mixed mode (purify and enrich mode run simultaneously even for 2 cell populations at a time).

For Analysis:

• Multicolor Immunophenotyping Characterization.
• Viability Assays.
• Cell Cycle and Proliferation Assays.
• Gene Expression (Fluorescent Protein analysis).
• Etc…