Techniques
- Transmission electron microscopy (TEM)
- Fixation methods for biological samples: chemical and/or cryofixation
- Dehydration and cryosubstitution techniques
- Embedding and cryoembedding
- Ultramicrotomy and cryoultramicrotomy techniques
- Negative staining techniques for suspensions
- Scanning electron microscopy (SEM)
- Fixation methods for biological samples: chemical and/or cryofixation
- Dehydration and cryosubstitution techniques
- Critical point drying of samples and other chemical agents
- Different types of assembly according to sample type
- Optical microscopy
- Semithin sections (0.5 – 3 μm) and specific stains
- Molecular localization techniques
- Immunolocalization techniques (markers: nanogold, gold, quantum dots, silver etc.)
- Lectin techniques (localization of sugar residues)
- Correlative techniques (Observation of the same sample with electronic and optical microscopes)
Range of services
- Transmission electron microscopy (TEM)
- Ultrastructural, tissue, cellular and molecular studies
- Studies of bacterial, viral, bacteriophagic and nitrogen suspensions, etc.
- Ultrastructural studies of isolated cell organelles, mitochondrial pellets and chloroplasts
- Whole-mount studies of cells and bacteria, etc.
- Molecular localization and co-localization of proteins, glucidic residues, etc.
- Cytochemical studies (Enzyme activity, digestion, etc.)
- Optical microscope studies with semithin sections of samples enclosed in resin
- Scanning electron microscopy (SEM)
- Microstructural analysis of cell cultures, tissues and organs with 3-D imaging
- Study of cell suspensions
- Surface studies: bone, teeth, corneas skin, etc.
- Study of biofilms and their formation on different surfaces
- Morphological studies of small organisms and microorganisms
- Molecular localization.
- Study of biomaterials, implants, etc.
- Study, analysis and evaluation of images obtained
Examples of application
- Study of cell cultures and tissues
- Study of fungal pathogens on plants
- Study of the incorporation of markers inside cells
- Study of human spermatozoa
- Studies of biofilms on water filters and cultures, tracheal tubes, historic moments, etc.
- Surface studies: prostheses removed from patients, presence of sperm on tissue
- TEM localization of nanogold in different tissues absorbed via inhalation in live rats
- Study of nanogold in textiles
- Study of bacteriophagic contamination in the bacterial production of lactose
- Study of protein aggregation in Alzheimer patients
- Clinical biopsy processing
Equipment
- Transmission electron microscope (TEM)
- Bioscan Gatan JEOL 1010 + transmission electron microscope
- Leica EM PACT high pressure cryofixation system
- Leica EMAFS cryofixation systems
- Reichert-Jung Ultracut E and Ultracut ultramicrotomes
- Leica Ultracut UCT ultramicrotome with Leica EMFCS cryoultramicrotomy unit
- Scanning electron microscope (SEM)
- Zeiss DSM 940 A digital scanning electron microscope
- Polaron critical point dryer
- Fisons Coating System SEM
Institution
Access Procedures
Any person applying for the first time for any services by CCiTUB must enter as a new user. For this purpose it is necessary to fill in an application form which can be downloaded from http://www.ccit.ub.edu/EN/m4.html. The form must be sent to
Keywords
Assurance of quality
ISO 9001:2008. The CCiTUB have well-established a system of management of the quality based on the ISO norm 9001, which also satisfies the Good Manufacturing Practice requirements (GMP).
Recognition by the Food and Drug Administration as a contract laboratory to perform drug tests.
Acknowledged laboratory Register of Agroalimentary Laboratories of Catalonia.