The DNA Capillary Sequencing Facility has provided support to researchers from public and private centers for more than twenty five years and has taken part in many national and international plant genome sequencing projects. We have acquired a remarkable experience on small and large scale DNA sequencing, which allows us to give full technical support and assessment to researchers. We use the platform ABI3730 DNA Analyzer for capillary electrophoresis and fluorescent dye terminator detection. We provide personalized service according to the user's needs.

Technique:

DNA sequencing and fragments analysis by automated capillary electrophoresis

Applications:

• De novo sequencing
• BAC-end sequencing
• Resequencing
• Heterozygote
• Genotyping: microsatellites, SNaPshot, SNPs, SNPlex, AFLP
• MicroRNA
• Sequencing of difficult templates
• Sequencing clean-up
• Quantification of nucleic acids

Equipment:

• 3730 ABI capillary electrophoresis DNA (48 capillary)
• Nanodrop 1000