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Cytometry

Techniques

  • Flow cytometry
  • Cell sorting

Range of services

  • Purification of cell populations: cloning, sorting of transfectants through flow cytometry (FACS) or magnetic separation (MACS)
  • Immunophenotyping of cell populations
  • Studies of cell viability and proliferation; high-throughput cell screening
  • Analysis of the cell cycle and ploidy variations
  • Cell function: measurements of second messengers, free-radical production, enzymatic kinetics
  • Plate immunoassays on microparticles
  • Detection and counting of microorganism populations

Examples of application

  • Purification of tumor stem cells
  • Cloning and purification of cells on 96-well plates; hybridoma sorting
  • Monitoring of blood populations undergoing ex vivo pharmacological treatment
  • Detection of toxicity and apoptosis; drug screening using cell lines
  • Detection of soluble cytokines in serum
  • Detection and counting of micro-organism populations in water (sea water, water from purification plants, industrial waste water, etc.)
  • Sorting of Arabidopsis seeds or Drosophilia embryos based on GFP expression levels

Equipment

  • Bellvitge Campus
    • Beckman Coulter MoFlo Astrios cell sorter
    • Miltenyi Automacs magnetic cell separator (AutoMACS)
    • Becton Dickinson FACSCanto II flow cytometer
    • FACScalibur flow cytometer
    • Fluostar optima microplate reader (bmg) with double injection system and temperature regulator
  • Diagonal Campus
    • Becton Dickinson Aria Fusion cell sorter
    • Becton Dickinson SORP Aria I cell sorter
    • Cytomation MoFlo legacy cell sorter
    • COPAS  sorter for cell aggregates and organisms between 200 and 500 µm
    • 2 Beckman Coulter Gallios flow cytometer  (3 lasers, 10 colors)
    • Beckman Coulter FC500-MPL flow cytometer (tube or 96 well plate reader)
    • Beckman Coulter Cyan flow cytometer (2 laser, 7 colors)
    • Coulter Epics XL flow cytometer
    • Compucyte LSC solid-state cytometer
    • Leica CM3050S cryostat
Dr Jaume Comas Riu
Head of Unit
934 034 654
Access Procedures

Any person applying for the first time for any services by CCiTUB must enter as a new user. For this purpose it is necessary to fill in an application form which can be downloaded from http://www.ccit.ub.edu/EN/m4.html. The form must be sent to .

Affiliations
Assurance of quality

ISO 9001:2008. The CCiTUB have well-established a system of management of the quality based on the ISO norm 9001, which also satisfies the Good Manufacturing Practice requirements (GMP).

  

Recognition by the Food and Drug Administration as a contract laboratory to perform drug tests.

Acknowledged laboratory Register of Agroalimentary Laboratories of Catalonia.