Techniques
- Flow cytometry
- Cell sorting
Range of services
- Purification of cell populations: cloning, sorting of transfectants through flow cytometry (FACS) or magnetic separation (MACS)
- Immunophenotyping of cell populations
- Studies of cell viability and proliferation; high-throughput cell screening
- Analysis of the cell cycle and ploidy variations
- Cell function: measurements of second messengers, free-radical production, enzymatic kinetics
- Plate immunoassays on microparticles
- Detection and counting of microorganism populations
Examples of application
- Purification of tumor stem cells
- Cloning and purification of cells on 96-well plates; hybridoma sorting
- Monitoring of blood populations undergoing ex vivo pharmacological treatment
- Detection of toxicity and apoptosis; drug screening using cell lines
- Detection of soluble cytokines in serum
- Detection and counting of micro-organism populations in water (sea water, water from purification plants, industrial waste water, etc.)
Equipment
Bellvitge Campus
- Beckman Coulter MoFlo Astrios cell sorter (4 lasers)
- Miltenyi Automacs magnetic cell separator (AutoMACS)
- Beckman Coulter Gallios flow cytometer (3 lasers, 10 colors)
- Becton Dickinson FACSCanto II flow cytometer (2 lasers, 6 colors)
- FACScalibur flow cytometer (2 lasers, 4 colors)
- Fluostar optima microplate reader (bmg) with double injection system and temperature regulator
Diagonal Campus
- 2 Becton Dickinson Aria Fusion cell sorter (4 laser, biohood)
- Becton Dickinson SORP Aria I cell sorter
- Beckman Coulter Gallios flow cytometer (3 lasers, 10 colors)
- Beckman Coulter Cyan flow cytometer (2 laser, 7 colors)
- Luminex MagPix
- Leica CM3050S cryostat
Dr Jaume Comas Riu (Diagonal Campus) / Dr. Esther Castaño Boldú (Bellvitge Campus)
Head of Unit
934 034 654/ 934 034 863
Institution
Access Procedures
Any person applying for the first time for any services by CCiTUB must enter as a new user. For this purpose it is necessary to fill in an application form which can be downloaded from http://www.ccit.ub.edu/EN/m4.html. The form must be sent to .
Keywords
Assurance of quality
ISO 9001:2008. The CCiTUB have well-established a system of management of the quality based on the ISO norm 9001, which also satisfies the Good Manufacturing Practice requirements (GMP).
Recognition by the Food and Drug Administration as a contract laboratory to perform drug tests.
Acknowledged laboratory Register of Agroalimentary Laboratories of Catalonia.