Techniques
- Wide-field fluorescence and transmitted optical microscopy
- Confocal laser microscopy
- Two-photon excitation microscopy (multiphoton)
- Processing and analysis of images
- Cell cultures and micromanipulation
Range of services
- Fluorescence and transmitted light multichannel and multidimensional (xyztc) images
- Multi-position and mosaic images
- Live cell microscopy, short courses
- Analysis of molecular proximity: fluorescence resonance energy transfer (FRET)
- Analysis of molecular dynamics: fluorescence recovery after photobleaching (FRAP), Inverse FRAP, fluorescence loss in photobleaching (FLIP) and photolabelling
- Intravital microscopy on live animals (high speed multiphotons)
- Infrared optical microscopy: multiphoton and second-harmonic generation
- Maintenance of cell lines for microscopy experiments
- Cell micromanipulation: microinjection
- Cellular and molecular labelling (transfection, staining)
- Image analysis:
- Image processing (filtered, 3D reconstruction, video montages) and analysis (co-localization,
- molecular dynamics, particle and surface analysis)
- Development of instruction macros
- Assistance in the design, preparation, performance and analysis of optical microscopy experiments
- Theoretical and practical courses
Examples of application
- Confocal profilometry
- Molecular imaging for the study of cellular and molecular processes in vivo
- Functionality of biosensors and drug delivery systems in cell cultures
- Cytotoxicity and cell viability trials
- Cell migration trials in 2D and 3D cultures
- Quantification of bone-implant contact (BIC) in teeth
- Morphometric analysis
- Subcellular and kinetic localization of fluorescent pharmaceuticals
- Neurite growth analysis
Equipment
- Culture room
- Fiji Image J, MetaMorph, Imaris and Huygens image treatment workstations
Diagonal Campus
- Carl Zeiss LSM 880 spectral confocal microscope
- Leica SPII confocal microscope with spectral detection
- Leica TCS-SPE spectral confocal microscope
- Leica DMIRB wide-field transmitted light and fluorescence microscope
- Olympus Fluoview confocal microscope
- Leica MLZIII stereo fluorescence microscope
Casanova Campus
- Carl Zeiss LSM 880 spectral confocal microscope
- Leica TCS-SL confocal microscope with spectral detection
- Leica TCS-SP5 high speed, multiphoton, spectral confocal microscope.
- Leica TCS-NT confocal microscope
- Leica DMI6000 B wide-field fluorescence microscope
- Leica ASTP microinjector
Bellvitge Campus
- Carl Zeiss LSM 880 spectral confocal microscope
- Leica TCS-SL confocal microscope with spectral detection
- Carl Zeiss SteREO Lumar V12 stereo fluorescence microscope
- Nikon E800 Microscope
- Leica DMIRB Microscope
Institution
Access Procedures
Any person applying for the first time for any services by CCiTUB must enter as a new user. For this purpose it is necessary to fill in an application form which can be downloaded from http://www.ccit.ub.edu/EN/m4.html. The form must be sent to
Affiliations
Keywords
Assurance of quality
ISO 9001:2008. The CCiTUB have well-established a system of management of the quality based on the ISO norm 9001, which also satisfies the Good Manufacturing Practice requirements (GMP).
Recognition by the Food and Drug Administration as a contract laboratory to perform drug tests.
Acknowledged laboratory Register of Agroalimentary Laboratories of Catalonia.