Next-generation sequencing (NGS) is becoming the reference of medical genomics. Our two next-generation sequencers, MiSeq (Illumina) and GS Junior (454 Roche), fulfil small-scale NGS project needs. We also offer Sanger sequencing and small/medium-throughput real-time PCR services, which continue to be highly required by users, and other minor DNA and RNA procedures.
Applications
Small genome de novo sequencing
Targeted or exome resequencing
ChIP-seq and small RNA-seq
Structural variation analysis
Targeted transcriptomics and metagenomics.
Ancient DNA sequencing
Genotyping, Gene expression, microRNA and Digital PCR analysis (real-time PCR)
Sanger sequencing and Fragment analysis
DNA, cDNA and RNA quantification and quality assessment
Robotic procedures for DNA normalization and PCR or sequence clean-up
Equipment
Next-Generation Sequencers:
- Illumina MiSeq
- 454/Roche GS Junior
Real-time PCR:
- LifeTechnologies Taqman Open Array
- 2 x ABI 7900HT (for user self-service)
- 2 x LightCycler 480 (for user self-service)
Capillary sequencers:
- ABI 3730 XL
- ABI 3130 XL
Others:
- Agilent Bioanalyzer
- QuantiFluor ST Fluorometer (Picogreen)
- Beckman Paradigm Detection Platform (Picogreen)
- Robotics (Beckman Biomek FX, Biomek NX and REMe)
PRBB building, 4th floor, lab 406 & office 421