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Confocal Microscopy Unit

The Confocal Microscopy Core Facility at Hospital Sant Joan de Déu is located in the Anatomical Pathology Service, which offers a wide array of routine and specialized pathology services for the study of cell biology, pathology and pathogenesis of diseases. Since its creation in 2017, one of the main objectives of the Confocal Microscopy Unit has been to explore, assess and improve the experimental as well as diagnostic abilities of this technology in the field of clinical and experimental Pathology in an excellent academic environment. The Confocal Microscopy Unit provides a complete range of light microscopy imaging systems to cover the whole spectrum of applications of conventional and advanced optical microscopy to both internal and external users. To make available this powerful but still poorly known new technology to the clinical specialists, the Unit covers, fully or partially, the processing of specimens until their observation by fluorescence or confocal microscopy, including application of immunolabelling techniques.

Applications

  • Nanoscopy imaging: STED 3X (Stimulated emission depletion 3D). Multicolor with 592, 660 and 775 STED depletion lines and gated-STED
  • Super-resolution microscopy: HyVolution
  • Multidimensional confocal imaging
  • Time-lapse fluorescence microscopy: tracking, intracellular calcium… Environmental control for in vivo imaging
  • High speed Confocal Microscopy
  • Colocalization and quantitative fluorescence studies
  • F-Techniques: FRET, FRAP, photoactivation...
  • Spectrum excitation and emission of molecules: lambda-square
  • Microscopy for multi-layered experiments and mosaics
  • Transmitted light microscopy: BF, DIC and polarized light
  • Conventional fluorescence microscopy
  • Image processing and analysis: LAS X, ImageJ, Fiji, Huygens

Technologies

The unit provides conventional and advanced light microscopy systems, equipment for sample preparation and maintenance prior to imaging, as well as resources for the data processing.

Available systems:

  • Confocal multispectral Leica TCS SP8 with a high speed module and a STED 3X module for super-resolution with 2 HyDs and Huygens STED deconvolution license. Laser lines: 405 nm, 488 nm and white laser for 470-670 nm excitation. Depletion lines: 592, 660 nm and 775nm.
  • Transmitted illumination and fluorescence widefield microscopy. Bright field, differential interference contrast (DIC), polarized light and fluorescence multiple wavelength imaging. Light upright microscope Leica DM5500B and DFC7000T digital color camera.
  • Computer workstations for fluorescence and confocal processing and analysis images (LAS X, Fiji / ImageJ, Huygens).
  • Auxiliary equipment for preparation and maintenance sample

Check rates, schedules and booking process

Mònica Roldán Molina
Coordinator
+34 932532145
Access Procedures

The facility is located in the Hospital Sant Joan de Déu, building “C”, floor 0, in the Anatomical Pathology Service. The address of the hospital is Passeig Sant Joan de Déu, 2. 08950 Esplugues de Llobregat (Barcelona, Spain).

The Unit is open to welcome scientists and clinicians from the Research Institut Sant Joan de Déu and Hospital, other public institutions or private companies.

Check rates and Booking regulation

The booking forms will be attended to by arriving order and via e-mail (mroldanm@sjdhospitalbarcelona.org). Please send a request describing the goal and principles of the experiments to be checked for feasibility at the site, additional requirements, as well as the expected scope and duration. We strongly advise that you book at least 15 days ahead of time. Late cancellations (< 24h in advance) will result in billing 50% of the fee.